Techniques in Biotechnology:
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AFLP’s:
Includes detailed information about AFLP. The webpage is subdivided into
Facts,
Interpretations, &
Further Info; can be a useful guide for students & people who would like
to understand AFLP’s at introductory level.
URL:
http://opbs.okstate.edu/~melcher/MG/MGW1/MG11127.html
Type: Webpage
Access: Free
URL:
http://ravel.zoology.wisc.edu/sgaap/AFLP_html/AFLP.htm
Type: Webpage
Access: Free
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BioGuide –
PCR: Answers questions like “What is PCR?, What are some good
reference books for PCR?, How should I select a set of primers to use for PCR?,
Programs for designing PCR primers, What is "Hot-start" PCR?, What is AP-PCR
or RAPD PCR?, What is "Touchdown" PCR? and Is there a simple method to
sequence lambda, M13, or plasmid clones using PCR?”
URL:
http://bioinformatics.weizmann.ac.il/mb/bioguide/pcr/PCRwhat.html
Type: Webpage
Access: Free
URL:
http://www.molbiol.ox.ac.uk/
Type: Website
Access: Free
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BioMedNet
– bmn.com:
It is an excellent website maintained by Elsevier.
Featured channels include – Journal Collection, Reviews, Research Update
Research Tools, News & Features, Books & Labware and Science Jobs. A must
visit for all life science related people.
URL:
http://www.bmn.com/
Type: Website
Access: Free (Partial),
registration required.
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Bionautics:
It is a
new web portal for molecular biology, genetics, and genomics. Links related to
DNA processing and analysis, Protein processing and analysis, Software on the
internet, etc can be seen. Other links include – Pairwise alignments, Plasmid
drawing, Backtranslation and Primer design.
URL:
http://entelechon.de/
Type: Website
Access: Free
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bioProtocol:
This is a part of
www.bio.com, dedicated to “Protocols & Tools” of Biological Sciences. The
available protocol disciplines include - Arabidopsis,C. elegans, Cell
Biology, Chromatin, Drosophila, Ethanol, In Focus Protocols Laboratory
Methods, Molecular Biology: Working with DNA, Molecular Biology: Working with
Proteins, Molecular Biology: Working with RNA, Plant Biology, Yeast, etc.
URL:
http://bioprotocol.bio.com/protocolstools/index.jhtml
Type: Website
Access: Free
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biowww dot
net - Lab Trouble Shooting Centre:
is “the first website collecting lab
bench work trouble shooting resources and protocols for researchers in
molecular biology, cell biology, immunology, genetics and biochemistry.”
“biowww.net is designed as a directory of lab benchwork trouble-shooting
resources to serve the needs of bioresearchers. It includes a comprehensive
collection of lab bench trouble-shooting web resources, which are mostly
collected from publically accessible bio-newsgroups and academic websites.”
URL:
http://www.biowww.net/
Type: Website
Access: Free
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Biochemistry, Molecular biology and Cell Biology protocols:
This is maintained by David Bowtell. Resources include - Isolation of DNA
fragments using glass milk (GENE-CLEAN), SOUTHERN BLOT, SOUTHERN ANALYSIS OF
MOUSE TOE/TAIL DNA, Northern transfer protocol, Hybridizing Northern blots
with riboprobes, cDNA + GENOMIC DNA LIBRARY SCREENING, Isolation of Mouse
genomic DNA, RNA ISOLATION USING GUHCL, POLY A+ RNA PREPARATION using
proteinase K and SDS, SELECTION OF POLYADENYLATED RNA FROM TOTAL RNA, Mini
plasmid preps, Large Scale Plasmid Preps, Preparing and USING bacterial cells
for electroporation, In situ hybridisation to RNA in tissue sections, PRIMER
LABELLING FOR PRIMER EXTENSION ASSAYand PRIMER EXTENSION, DNA-se I
FOOTPRINTING, etc. A must visit for all those who work in the field of
molecular biology
URL:
http://grimwade.biochem.unimelb.edu.au/sigtrans.html
Type: Webpage
Access: Free
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Brendan
Babb's Collection of Protocols and Recipes:
This is an
excellent resource webpage giving molecular biology techniques, protocols and
recipes. Webpage is maintained by Brendan Babb at the department of Medical
Microbiology, University of Cape Town. Protocols include - Bacterial methods,
DNA related protocols, RNA related protocols, Protein related protocols,
Polymerase Chain Reaction, etc. Apart from this an exhaustive alphabetical
listing of Recipes is also seen. Excellent and must visit for molecular
biologists.
URL:
http://www.uct.ac.za/depts/mmi/bbhelp/int.html
Type: Webpage
Access: Free
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Chen’s
Protocol List: Contains protocols like – “Preparation of competent
cells, Agarose gel electrophoresis, PCR, primer design, DNA digest, Ligation,
Screening for recombinants, Mutagenesis, Phenol/chloroform, DNA preps, DNA
Sequencing” etc. A good collection external links also available.
URL: http://www.biochem.ucl.ac.uk/~chen/index.html
Type: Webpage
Access: Free
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Dolan DNA
Learning Center (DNALC):
“The goals of the DNA Learning Center
are: “To serve as a clearinghouse for information on DNA science, genetic
medicine, and biotechnology, To provide an interactive learning environment
for students, teachers, and nonscientists, To explore and develop new
instructional technologies to make DNA science accessible to the public, and
especially, young people” etc. Lots of resources related to DNA science,
genetic medicine, and biotechnology are available.
URL: http://www.dnalc.org/
Type: Webpage
Access: Free
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Donis-Keller
Lab Manual: Contents include - Lambda Phage Propagation and DNA
Preparation Methods, Plasmid/Cosmid Propagation and DNA Preparation, Yeast
Artificial Chromosome (YAC)Methods , Human DNA Preparations, Restriction
Digestion Methods, Southern Transfer and Hybridization, DNA Sequencing, PCR
Methods, Denaturing Gradient Gel Electrophoresis (DGGE), Human Cell Culture
Methods, Chromosome Analysis Methods
URL:
http://hdklab.wustl.edu/index.html
Type: Webpage
Access: Free
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Highveld.com:
It is “the Internet Directory of Molecular Biology and
Biotechnology. The scientist's guide to molecular biology databases, molecular
biology protocols, molecular biology journals, molecular biology news,
molecular biology information, and all molecular biology related resources.”
“Maintained by a team of expert science editors, our pages are packed full of
links and information on all aspects of molecular biology and biotechnology.
Highveld.com is a major
Information Resource Center for Molecular Biologists and Biotechnologists.”
Links include – Protocols, PCR, Genomics, Journals, Books, Microbiology,
Genetics, Molecular Biology, Jump Stations, Probiotics, PNA, Ribozymes, NMR,
Genomes, Databases, Newsgroups, Proteomics, etc.
URL:
www.highveld.com
Type: Website
Access: Free
URL:
http://iprotocol.mit.edu/
Type: Webpage
Access: Free
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MAKING PCR:
A document hosted on
http://sunsite.berkeley.edu/ server, it tries to answer questions like
what is the polymerase chain reaction, what are the current trends in PCR
research, etc. It also contains information about the PCR history; links
included in this section are - PCR HISTORY: FOUNDATIONAL PAPERS, ORAL HISTORY
OF ARTHUR KORNBERG M.D., etc.
URL:
http://sunsite.berkeley.edu/PCR/whatisPCR.html
Type: Document
Access: Free
URL:
http://www.bioinformatics.vg/index.shtml
Type: Website
Access: Free
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Molecular
Cloning –
A Laboratory Manual: Authored by Joseph Sambrook and
David W. Russell, this online manual is “essential information source for
laboratories working with DNA and the Internet is now an essential means of
delivering scientific information. MolecularCloning.com contains summarized
versions of protocols from the third edition of Molecular Cloning: A
Laboratory Manual, published in December 2000. The abbreviated protocols can
be searched by keyword, downloaded, and printed out in a format that makes
them usable as guides when working through methods at the lab bench.”
URL:
http://www.molecularcloning.com/index.html
Type: Website
Access: Free
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MolBioSpot:
The webpage contains a list of links related to web tools for molecular
biologists, specifically DNA/Protein sequence analyses. List includes Most
popular servers, Search for transcription factor binding-sites, Prediction of
splice sites and ORFs, Restriction mapping, Protein utilities, etc. Apart from
this a large number of links about the protocols can also be found.
URL:
http://www.geocities.com/richyuhk/
Type: Webpage
Access: Free
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Molecular
Biology Protocols:
“Mark Strom at the Microbial
Pathogenesis/Utilization Research Division, Northwest
Fisheries Science Center (NMFS/NOAA) maintains this excellent collection of
molecular biology protocols. He also maintains a useful Molecular Biology
Techniques Forum where you can submit tips, techniques, questions and
comments. The methods are nicely organised into sections, including a section
on computer analysis of data.” Links include - DNA Purification Techniques,
DNA Transformation/Library Preparation, Southern/Northern Blotting, DNA
Sequencing, Oligonucleotides, PCR and Related Methods, RNA Methods, Protein
Electrophoresis, Protein Purification, Autoradiography Tips, Safety, etc.
URL:
http://micro.nwfsc.noaa.gov/protocols/index.html
Type: Webpage
Access: Free
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Molecular
Biology Methods:
Maintained by Bruce A. Roe, Department of
Chemistry and Biochemistry, University
of Oklahoma, Norman, Oklahoma, this manual contains many methods used in
molecular biology laboratory. The links contain – DNA sequencing protocols and
DNA sequencing automation techniques. Other links include general methods,
Random subclone generation, Methods for DNA isolation, Methods for DNA
sequencing, Additional methods, etc.
URL:
http://www.genome.ou.edu/protocol_book/protocol_index.html
Type: Webpage
Access: Free
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PCR and
multiplex PCR guide:
Maintained by Octavian Henegariu, the webpage
contains information about PCR and related information; links include PCR
guide, PCR troubleshooting, Standards, Applications, etc.
URL:
http://info.med.yale.edu/genetics/ward/tavi/PCR.html
Type: Webpage
Access: Free
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Practical
Molecular Biology:
It “is a non-profit project, which aims to help
people involved in molecular biology research get together and assist each
other”. Protocols sections includes a large list of recipes for salt
solutions, laboratory buffers, media, antibiotics, etc. Also included are the
links related to calculations like weight-to-molar converters of protein and
nucleic acid concentrations, the [rpm]-to-[g] converter of centrifugation
rates, the Time Estimation tool for bacteria growth, etc.
URL:
http://www.molbiol.ru/eng/
Type: Website
Access: Free
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Protocols
related to genomics:
C. elegans Genome, C. briggsae
Genome Human Genome, Mouse Genome, Nematode.net, EST Projects, Bacterial
Genomes, Histoplasma Capsulatum, Chimpanzee Genome, Chicken Genome, etc.
URL:
http://genome.wustl.edu/tools/protocols/index.php
Type: Webpage
Access: Free
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PCR
Links.com:
An excellent resource website; information about
almost all PCR related techniques. Links include - PCR, AFLP, Alu-PCR,
Asymmetric PCR, Colony PCR, DD-PCR, Degenerate PCR, Hot-start, In situ PCR,
Inverse PCR, Long-PCR, Multiplex PCR, Nested PCR, PCR-ELISA, PCR-RFLP,
PCR-SSCP, QC-PCR, RACE, RAPD, Real-Time PCR, Rep-PCR, RT-PCR, etc.
URL:
http://www.pcrlinks.com/
Type: Webpage
Access: Free
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PCR
Protocols: Includes Quantitative Reverse Transcription
Polymerase Chain Reaction (RT-PCR) and Other PCR Procedures. Maintained by
Department of Veterinary Science and Molecular Toxicology Program, Penn State
University, this website tries to explain PCR with following headings -
SEARCHING THE DATABASE FOR SEQUENCE OF INTEREST, PRIMER SELECTION, OPTIMIZING
PRIMER SET, MAKING OF A PCR INTERNAL STANDARD and QUANTITATIVE RT-PCR. Also
includes the links BASIC RT-PCR RECIPES, BUFFER AND REAGENT COMPOSITIONS,
etc.
URL:
http://www.cas.psu.edu/docs/CASDEPT/VET/jackvh/jvhpcr.html
Type: Document
Access: Free
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PCR @
Molecular Biology Techniques Manual:
Edited by Vernon E Coyne, M
Diane James, Sharon J Reid and Edward P Rybicki, PCR is dealt under following
subheadings – PCR PRIMER DESIGN AND REACTION OPTIMISATION, STANDARD PCR
PROTOCOL, REVERSE TRANSCRIPTION PCR, CORE SAMPLE PCR and CALCULATING
CONCENTRATIONS FOR PCR.
URL:
http://web.uct.ac.za/microbiology/manualin.htm
Type: Webpage
Access: Free
URL:
http://hdklab.wustl.edu/lab_manual/index.html
Type: Webpage
Access: Free
URL:
http://www.accessexcellence.com/AB/BC/PCR_Xeroxing_DNA.html
Type: Webpage
Access: Free
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Protocols
Used in the Roe Laboratory:
A part of Roe Lab Protocol Book, the
webpage includes links on Gel Preparation Protocols, Protocol for Enhancing
PCR of Very Difficult Regions, Protocol for Sequencing Very Difficult Regions,
etc. Other important links include - Uniplex PCR-based Sequencing, MultiPlex
PCR-based Sequencing, Cloning MultiPlex PCR-based Products for DNA Sequencing
in pUC-based Vectors, etc.
URL:
http://www.genome.ou.edu/protocol_book/protocol_index.html
Type: Webpage
Access: Free
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RAPD:
Maintained
by Ulrich Melcher, Agricultural Sciences and Natural Resources division,
Oklahoma State University, this webpage gives basic methodology employed in
RAPD technique; a good resource guide for beginners. Other links include - PCR
amplification, genetic mapping, chromosome walks, RFLP's, SSCP's, AFLP's and
VNTR's.
URL:
http://opbs.okstate.edu/~melcher/MG/MGW1/MG11127.html
Type: Webpage
Access: Free
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RTPrimerDB
–
The Real Time PCR Primer and Probe Database: “This is a public
database holding real time PCR primers and probes for popular chemistries
(Intercalating Dyes, Hydrolysis Probes, Hybridization Probes, and Molecular
Beacons) to prevent time-consuming primer design and experimental
optimization.” As of today about 159 people have contributed over 793 primer
sets.
URL:
http://medgen31.rug.ac.be/primerdatabase/index.php
Type: Webpage
Access: Free & Interactive
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SSCP's:
Maintained
by Ulrich Melcher, Agricultural Sciences and Natural Resources division,
Oklahoma State University, this webpage gives basic methodology employed in
SSCP technique; a good resource guide for beginners. Other links include –
RAPD, PCR amplification, genetic mapping, chromosome walks, RFLP's, SSCP's,
AFLP's and VNTR's.
URL:
http://opbs.okstate.edu/~melcher/MG/MGW1/MG11129.html
Type: Webpage
Access: Free
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Standard
PCR Protocol:
Edited by Vernon E Coyne, M Diane James, Sharon J Reid
and Edward P Rybicki, this document contains links like Recommended Reagent
Concentrations, Recommended Reaction Conditions, Initial Conditions,
Temperature Cycling, "Hot Start" PCR, Asymmetric PCR for ssDNA Production,
Detecting Products, Labelling PCR Products with Digoxigenin, Cleaning PCR
Products, Sequencing PCR Products, Cloning PCR Products, etc.
URL:
http://web.uct.ac.za/microbiology/pcrcond.htm
Type: Webpage
Access: Free
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Tavi’s
FISH Protocols and Slide Preparation Guide:
Maintained by Octavian
Henegariu, this webpage contains information related to FISH & related
techniques. It contains two broad subheadings - Slide preparation guide and
FISH guide, which contain detailed descriptions about the techniques involved.
Other links include – PCR, dUTP label, CCK, Slide prep, CM-FISH, TM-FISH,
mArrays, etc.
URL:
http://info.med.yale.edu/genetics/ward/tavi/FISHguide.html
Type: Webpage
Access: Free
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Which DNA
Marker for Which Purpose?:
It is the “Final Compendium of the
Research Project Development, optimisation and validation of molecular tools
for assessment of biodiversity in forest trees in the European Union DGXII
Biotechnology FW IV Research Programme Molecular Tools for Biodiversity”;
includes links like Which DNA Marker for Which Purpose?, Sampling Strategies
for Marker Analysis and Software for Marker Analysis; a good resource guide.
URL:
http://webdoc.sub.gwdg.de/ebook/y/1999/whichmarker/cmarker.htm
Type: Webpage
Access: Free
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